Journal: Journal of Physiology and Biochemistry

Article Title: Modulation of SIRT1/PPARγ pathways and tight junction proteins by nicotinamide riboside under chronic variable stress

doi: 10.1007/s13105-026-01153-7

Figure Lengend Snippet: The effects of different nicotinamide riboside doses on the levels of Claudin-1 ( A ), Claudin-4 ( B ), Occludin ( C ), ZO-1 ( D ), MUC-2 ( E ), SGLT1 ( F ), and GLUT2 ( G ) proteins in the jejunum tissue of rats under normal (N) and chronic variable stress (CVS) conditions. Western blot analysis was performed using β-actin as a loading control to ensure equal protein loading. All bands are shown in ( H ). Different letters (a–e) indicate statistically significant differences between groups ( p < 0.05) according to Tukey’s post hoc test following two-way ANOVA. Two-way ANOVA revealed significant effects for Claudin-1 (Condition p < 0.0001; NR p < 0.0001; Condition x NR p = 0.0002), Claudin-4 (Condition p < 0.0001; NR p < 0.0001; Condition x NR p = 0.0155), Occludin (Condition p < 0.0001; NR p < 0.0001; Condition x NR p < 0.0001), ZO-1 (Condition p < 0.0001; NR p < 0.0001; Condition x NR p < 0.0001), MUC-2 (Condition p < 0.0001; NR p < 0.0001; Condition x NR p < 0.0001), SGLT1 (Condition p < 0.0001; NR p < 0.0001; Condition x NR p = 0.0002), and GLUT2 (Condition p < 0.0001; NR p < 0.0001; Condition x NR p < 0.0001). Comprehensive statistical data are presented in Supplementary Table and full immunoblots images are shown in supplementary Fig.

Article Snippet: Membranes were blocked with 5% bovine serum albumin (BSA) for 2 h at room temperature and incubated overnight at 4 °C with primary antibodies as follows: PPARγ (sc-271392), GLUT2 (sc-518022), IRS-1 (sc-8038), SIRT1 (sc-74465), FASN (sc-48357), claudin-1 (sc-166338), claudin-4 (sc-376643), occludin (sc-133256), ZO-1 (sc-33725), MUC2 (sc-53381), PepT1 (sc-373742), LAT2 (sc-293242), FABP2 (sc-374482), FATP4 (sc-393309) and β-Actin (sc-517582) (all from Santa Cruz Biotechnology); SGLT1 (DF7202-100; Affinity Biosciences); EAAT3 (orb1161904; Biorbyt Ltd., Cambridge, UK).

Techniques: Western Blot, Control

Journal: Molecular Therapy. Methods & Clinical Development

Article Title: Efficient kidney gene transfer and proximal tubule transduction using self-complementary AAV.cc47 vectors

doi: 10.1016/j.omtm.2025.101606

Figure Lengend Snippet: AAV.cc47 expresses in early proximal tubule segments (A) Schematic of kidney nephron and further subdivision of the proximal tubule, which can be separated by Sglt1 (proximal straight tubule, yellow) and Sglt2 (proximal convoluted tubule, cyan) expression. Immunofluorescence of (B) whole kidney and (C) inserts of mouse kidney sections from mice injected intravenously with sham or 1 × 10 12 vg of scAAV.cc47-CBh-mCherry and harvested after 3 weeks. n = 3 mice per group. Sections stained with DAPI (nuclei, gray), mCherry (viral transgene, magenta), Sglt2 (proximal convoluted tubule, cyan), and Sglt1 (proximal straight tubule, yellow). (D) mCherry H-score was quantified through previously outlined QuPath automated detection and analysis. H-score was determined through the sum of staining intensity (1–3) multiplied by the percentage of positively stained cells for that intensity, with possible scores ranging from 0 to 300. Both kidneys were quantified for each mouse. The classifier was trained to identify glomeruli, Sglt1+ cells, Sglt2+ cells, and non-glomerular or proximal tubular cells as “other.” (E) Quantification of cell type identification for all transduced cells detected. Scale bars, 1 mm in (B) and 100 μm in (C).

Article Snippet: Primary antibodies used were mCherry (1:500; rat; Invitrogen M11217 ), Oat1 (1:1,000; mouse; LS Bio LC-C209560, Newark, CA), RFP (1:500; rabbit; MBL PM005, Woods Hole, MA), Sglt1 (1:1,000; rabbit; Izumi Kaji lab 576–610, Nashville, TN), and Sglt2 (1:100, mouse; Santa Cruz sc-393350, Dallas, TX).

Techniques: Expressing, Immunofluorescence, Injection, Staining